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KMID : 0352819960110010019
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Kosin Medical Journal
1996 Volume.11 No. 1 p.19 ~ p.30
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Insulin and IGF-I Stimulate the Growth of Human Pancreatic Cancer Cells in vitro
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Abstract
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Background/Aims:
@EN Insulin and IGF-I have trophic effects on a variety of tissue cells, and are known to increase protein and enzyme synthesis of pancreatic acinar cells. The purpose of this study is to asses the effects of insulin and IGF-I on the growth
of
human pancreatic cancer cells(Mia PaCa-2 and CAPAN2).
@ES Methods:
@EN Mia PaCa-2; 48h after platine cells(5-10×104 cells/well), medium was changed to serum-free medium.48h after changing medium, insulin(0.0-1.00 ug/ml) or IGF-I(0.1-100.0 ng/ml) was added. Cells were counted 4 days after
treatment.
In another experiment, IGF-I receptor antibody(aIR-3) was added(5000 ng/ml) with or without insulin(1.0 ug/ml).CAPAN2; The experiment procedure was the same except cell number(10-20×104 ccells/well) and day of cell counting(5 days).
aIR-3
was
added with or without insulin(1.0 ug/ml) or IGF-I(100.0 ng/ml). All experiments were repeated at least twice.
@ES Results:
@EN 1) Insulin and IGF-I stimulated the growth of both well differentiated(CAPAN2) and undifferentiated(Mia PaCa-2) pancreatic cancer cell lines. 2) The concentrations at which insulin stimulated the growth of mia PaCa-2 and CAPAN2
were
100.
ng/ml and 1000 ng/ml, respectively, which showed marked differences according to different cell lines. In contrast, IGF-I stimulated the growth of Mia PaCa-2 and CAPAN2 at the same concentration, viz 10.0 ng/ml. 3) aIR-3(5000 ng/ml)
inhibited
the growth-stimulatory effect of insulin(1.0 g/ml) on Mia PaCa-2 cells.
@ES Conclusions:
@EN Insulin and IGF-I have growth-stimulatory effects on pancreatic cancer cells, and this effect may be exerted through different receptors according to different cancer cells. IGF-I receptor antibodies may become a useful anticancer agent
for
pancreatic cancer.
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KEYWORD
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